Coding

Part:BBa_K1796102:Design

Designed by: Tang Yulong   Group: iGEM15_SCU_China   (2015-09-17)


A chain of CODH/ACS beta subunit


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 2042
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 2043
    Illegal PstI site found at 2057
    Illegal NotI site found at 7
    Illegal NotI site found at 2050
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 2043
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 2043
    Illegal PstI site found at 2057
    Illegal AgeI site found at 341
    Illegal AgeI site found at 746
    Illegal AgeI site found at 971
    Illegal AgeI site found at 1046
    Illegal AgeI site found at 1511
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1311


Design Notes

Codon preference has been changed to suit E.coli. And,some restriction sites have been avoid.


Source

This parts is cloned from the genomic sequence of Moorella thermoacetica

References