Coding
Part:BBa_K1796102:Design
Designed by: Tang Yulong Group: iGEM15_SCU_China (2015-09-17)
A chain of CODH/ACS beta subunit
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 2042 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal SpeI site found at 2043
Illegal PstI site found at 2057
Illegal NotI site found at 7
Illegal NotI site found at 2050 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
- 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 2043 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 1
Illegal XbaI site found at 16
Illegal SpeI site found at 2043
Illegal PstI site found at 2057
Illegal AgeI site found at 341
Illegal AgeI site found at 746
Illegal AgeI site found at 971
Illegal AgeI site found at 1046
Illegal AgeI site found at 1511 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1311
Design Notes
Codon preference has been changed to suit E.coli. And,some restriction sites have been avoid.
Source
This parts is cloned from the genomic sequence of Moorella thermoacetica